Background: Since the outbreak of the COVID-19 pandemic, the acceptance of the coronavirus-19 vaccine has been one of the most indispensable steps of the world's mission to confront the disease. Hence, acceptance and awareness of COVID-19 vaccination and third dose among the public were measured. This study was carried out with an objective to assess the quality of web-based health information on COVID-19 and the willingness to accept the third dose among citizen of the Riyadh region. Methodology: A cross-sectional online survey was conducted to measure the need for a booster dose and it’s acceptance and the quality of web-based information. A total of 631 people contributed their responses. All males and females above 18 years of age living in the Riyadh region of Saudi Arabia were included in the study. This research was conducted in randomly chosen Riyadh, Al Majma'ah, Al Ghat, and Zulfi cities of Riyadh Region. Results: A highly significant association (p<0.001) between the awareness of vaccine effectiveness and trust in the ability of the vaccine to prevent complications and acceptance of the third dose was observed. Almost 54.8% participants accepted to take the booster dose regardless of any belief about the side effects of COVID-19 vaccines. Conclusion: The acceptance of a COVID-19 third dose vaccine was higher in females, single individuals, those aged 18-24, and those with a bachelor's or a diploma degree, and it revealed a positive association between those who had previously been infected with COVID-19 and acceptance of the booster dose. It has shown that many people depend on the health ministry as the first source for obtaining health information about covid19.