*Corresponding Author:
Dr. EC Ojiyi
Department of Obstetrics and Gynecology, Imo State, University Teaching Hospital, Orlu, Imo State, Nigeria
E-mail: [email protected]

Abstract

Background: Infection with human papilloma virus (HPV) is the main cause of cervical cancer, but the local risk factors have not been sufficiently assessed. Aim: The study is aimed at determining the prevalence and to evaluate the local risk factors of HPV infection in cervical smears at the Imo State University Teaching Hospital, Orlu, Nigeria. Subjects and Methods: The participants involved 445 randomly selected sexually active women attending the antenatal, postnatal, gynecology and family planning clinics in the Department of Obstetrics and Gynecology of the university between April 2004 and May 2012. A questionnaire assessing various socio‑demographic characteristics of the participants was administered. The pap smears of the participants were examined microscopically for evidence of HPV infection. The SPSS version 17.0 (Chicago, Illinois, USA) was used to compute and analyze the results. The results were presented in tables as simple percentages. Tests of significance using the Chisquare and fisher exact tests were applied where appropriate. Results: The prevalence rate of HPV was 10.3%. The peak age‑specific prevalence of 11.7% occurred in the 15‑19 years age group. There were significant associations between the occurrence of HPV and multiple sexual partners, coital frequency, multiparity, contraceptive use, marital status, low socio‑economic status, abnormal vaginal discharge, irregular menstruation, post‑coital and post‑menopausal bleeding, (P < 0.05). Conclusion: All sexually active women including teenagers should be screened for cervical HPV infection in an organized systematic program equipped with a good call and recall system. There is, therefore, a need to move emphasis from the current practice of opportunistic screening to a systematic screening of the whole population at risk despite cost implications.

Keywords

Genital human papilloma virus, Pap smear, Risk factors

Introduction

Cervical cancer is a preventable disease, but has continued to pose a huge management challenge to the modern day gynecologist. It is the leading cause of death per annum in women aged 35-45 years.[1]

On worldwide basis, cervical cancer constitutes approximately 6% of all cancers in women.[2] An estimated 500,000 new cases are diagnosed every year with about 234,000 deaths.[2] It is the principal cancer of women in most developing countries, where 80% of cases occur, and which have only 5% of global cancer resources.[2,3] It was the most common malignancy amongst women in Nigeria and the rest of sub-Saharan Africa with a very poor survival rate.[1-5] It accounted for 63.1% and 66.2% of histologically confirmed gynecological cancers in Ilorin [2] and Zaria,[4] respectively, in Nigeria.

In 1842, Rigoni-Stern formally hypothesized that cervical cancer had an infective sexually transmitted etiology.[6] Many studies since then confirmed the veneral nature of cervical cancer and identified other risk factors. Molecular epidemiologic evidence clearly indicates that certain types of HPV are the principal cause of invasive cancer and cervical intraepithelial neoplasia.[3]

The HPV has been shown to be a determinant of the natural history of cervical intraepithelial neoplasia (CIN). The progression rate to cervical cancer when HPV co-exists with CIN is about 21% but only 5.6% when CIN lesions occur alone.[7]

Many studies have tried to show some kind of association between age, reproductive factors, marital factors, educational level, religion, occupation, and sexual behaviors with the risk of developing HPV and cervical cancer.[8] Such socio-demographic factors may be useful in risk scoring. This is important because risk scoring systems have the potential for assisting the targeting of screening resources, as broad-risk targeting of all sexually active women is not a viable option for developing countries due to paucity of both human and financial resources. Even in the industrialized nations of the West, the need for more precise targeting of high-risk groups in order to improve the efficiency of cytology programs and conserve funds has become a major issue.[9]

Sub-clinical HPV infections of the cervix may be diagnosed by colposcopy, viral DNA hybridization, polymerase chain reaction (PCR) amplification, histology or by the characteristic HPV changes on Papanicolaou smear.[8,10] The Papanicolaou smear for cervical cytology fulfills all the criteria for an ideal screening test. Not only is it cost-effective, acceptable to most patients, and adoptable to wide-spread screenings, it is specific enough to detect changes and subsequent progression to CIN resulting in decreased morbidity and mortality from invasive cervical cancer.[11] DNA hybridization and PCR amplification can detect both productive and non-productive infection but appear to be of limited value in predicting the risk of developing CIN or invasive carcinoma.[10]

Although cervical cytology, histology and colposcopy are less sensitive, they are capable of detecting significant pathological changes associated with productive HPV infection. The newer HPV detection procedures like the PCR, viral load, DNA hybridization, etc. merely detect the presence of the HPV virus in the tissues, but do not show whether the virus has started causing cellular changes that will eventually culminate in cervical cancer, unlike cytology, histology and colposcopy.[7,8] The aim of this study was to determine the prevalence of human papilloma virus infection in cervical smears taken in women attending gynecological, antenatal, postnatal, and family planning clinics at the Imo State University Teaching Hospital, Orlu and also to determine the epidemiological factors predictive of human papilloma virus infection of the cervix.

Subjects and Methods

This was a Prospective descriptive study involving 445 randomly selected sexually active women attending the antenatal, postnatal, gynecology and family planning clinics in the Department of Obstetrics and Gynecology of the Imo State University Teaching Hospital, Orlu, Nigeria between April 2004 and May 2012. They were recruited after consenting to participate, and a formal approval had been given by the institution’s Ethics and Research Committee. The recruitment continued until a sample size of 445 was reached.

This was calculated using the WHO Epi Info Version 6 program for population sampling.

It was based on a population of 4,261 patients/clients attending the recruiting clinics from April 2004 to May 2012. The expected frequency was 2.34%, the mean of reported studies from other parts of Nigeria.[10,11,12] The minimum acceptable result was taken to be 1.0%. The sample size required at 95% confidence interval was 439 using the formula:

Sample size = n/[1-(n/population)], where n = z*z [P {1-P}]/ (D*D).

The inclusion criteria were all sexually active women attending the above clinics and who consented to participate in the program were included until a sample size was reached. The exclusion criteria were women who declined to consent were exempted, so were those who had never been sexually exposed. In addition, those with obvious cervical warts, fungating, or ulcerative lesions of the cervix from which biopsy may be grossly directed were excluded.

Methods

The purpose, value of the procedure was explained to each prospective patient and her consent sought. All consenting patients had their Pap smears taken using a moistened and unlubricated Cusco’s bivalve speculum and an Ayre’s wooden spatula after a questionnaire on socio-demographics and sexual attitudes had been filled. The smears were transported to the histopathology laboratory immersed in 95% ethanol for preparation, staining and microscopy reading. The HPV produces characteristic cellular changes when it starts multiplying within the cells of the cervix. These inclusion bodies are called koilocytes and their presence within the cells of the cervix on microscopic examination after proper staining is pathognomic of HPV infection.[7,8]

Statistical analysis

The Statistical package for social sciences Version 17.0 (Chicago, Illinois, USA) was used to compute and analyze the results. These included frequency distribution and tests of significance using Chi-square (χ2). The Fisher Exact test was applied where appropriate.

Results

Four hundred and forty-five sexually active women attending various clinics at the Department of Obstetrics and Gynecology, of the Imo State University Teaching Hospital had their Pap smears taken and questionnaires on sexual attitudes, socio-demographics, and symptomatology filled.

Abnormal smears occurred in 238 (53.5%) of the patients screened. Forty-six women (10.3%) had HPV associated with changes, constituting 19.3% of all abnormal smears.

The ages of the participants ranged between 15 and 59 years, with a mean of (SD) 25.8 (4.6). Majority of the patients (65.9%) [293/445] were aged between 20-29 years. They were mostly married (66.1%) [294/445] and parous (69.7%) [310/445], but 24.9% [111/445] had no formal education. There were 429 (96.4%) Christians, while 33% [147/445] either had no occupation or were simply housewives.

Most (69.7%) [301/445] of the participants did not practice any form of contraception; the 30.3% [144/445] who did virtually used hormones (combined pills, progesterone only pills, implants, injectables) or intra-uterine contraceptive device only.

When the various factors studied were individually matched with HPV-associated changes, variable patterns of association were observed. Table 1 shows that there was no significant association between the participants’ age and HPV infection (P = 0.91).

Age (years) Positive Negative Total
15-19 7 53 60
20-24 17 140 157
25-29 13 123 136
30-34 5 47 52
=35 4 36 40
Total 46 399 445

Table 1: Association between age and human papilloma virus infection of the cervix

Table 2 shows the association between various sexual attitudes and HPV infection. There was a statistically significant association between multiple sexual partners, coital frequency per week, and the occurrence of genital HPV infection. In Table 3, statistically significant association is shown to exist between multiparity (P = 0.01), contraceptive use (0.02), duration of contraceptive use (P = 0.04), and genital HPV infection.

Variable Positive Negative Total
Age (years) at first coitus      
12-15 9 131 140
16-19 25 200 225
≥20 12 68 80
Total 46 399 445
χ2 for linear trend=0.01, df=1, P=0.92    
Duration (years) of sexual      
exposure      
≤10 21 241 262
11-20 14 123 137
≥21 11 35 46
Total 46 399 445
χ2 for linear trend=0.39, df=6, P=0.53    
Coital frequency per week      
1-2 23 289 312
3-4 17 101 118
≥5 6 9 15
Total 46 399 445
χ2=6.80, df=2, P=0.03      
Number of sexual partners      
1 8 124 132
≥2 38 275 313
Total 46 399 445
χ2=4.39, df=2, P=0.03      
Previous PID*/STD** treatment      
Yes 30 285 315
No 16 114 130
Total 46 399 445
χ2=4.29, df=2, P=0.41      

Table 2: Association between sexual attitudes and HPV infection of the cervix

Variable Positive Negative Total
Parity      
Non-parous 5 138 143
Parous 41 261 302
Total 46 399 445
χ2=3.34, df=1, P=0.01      
Contraception      
None 8 302 310
Hormones 21 86 107
Intrauterine 15 9 24
contraceptive device      
Others 2 2 4
Total 46 399 445
χ2 for linear trend=83.22, df=3, P=0.02    
Duration (years) of      
contraceptive use      
<1 3 59 62
1-5 15 23 38
≥6 18 8 26
Total 36 90 126
χ2 for linear trend=34.20, df=1, P=0.04

Table 3: Association between reproductive factors and HPV infection of the cervix

Table 4 shows that there was a statistically significant association between marital status (P < 0.001), occupation (P < 0.001), and genital HPV infection.

Variable Positive Negative Total
Marital status      
Single 18 10 28
Married 6 288 294
Widowed 8 24 32
Divorced 14 77 91
Total 46 399 445
χ2 for linear trend=76.68, df=2, P=0.01    
Number of marriages      
First marriage 4 118 122
Two and more marriages 7 164 171
Total 11 282 293
Fisher exact test=0.59, df=2, P=0.42    
Type of marriage      
Monogamy 3 107 110
Polygamy 8 175 183
Total 11 282 29
Fisher exact test=1.74, df=2, P=0.22    
Educational level      
None 9 104 113
Primary 8 59 67
Secondary 18 168 19
Post-secondary 11 68 79
Total 46 399 445
χ2 for linear trend=0.86, df=2, P=0.41    
Religion      
Christianity 41 388 429
Islam 5 11 16
Total 46 399 445
χ2=2.99, df=2, P=0.01      
Occupation      
Single, unemployed 2 14 16
Civil servant 4 97 101
Petty trader 13 59 72
Housewife 3 128 131
Student 11 20 31
Professional 4 39 43
Apprentice 8 16 24
Business executive 1 26 27
Total 46 399 445

χ2 for linear trend=26.11, df=5, P=0.01

Table 4: Association between marital status, number of marriages, educational level, religion, occupation, and HPV infection of the cervix

HPV infection was significantly associated with abnormal vaginal discharge (P < 0.001) and irregular menstruation (P = 0.01), as well as post-menopausal (P = 0.02) and post-coital bleeding (P < 0.001) as shown in Table 5. There was no significant association with vaginal itching (P = 0.46).

Discussion

The prevalence rate of HPV infection of the cervix of 10.3% (103 per 1000) in this study is fairly high. It is higher than the 4.01%,[12] 1.8%,[13] and 1.2% [14] reported in Ibadan, Maiduguri, and Enugu, respectively, but lower than the 48% [15] and 32% [16] reported in Brazil and Tanzania, respectively. The differences in all these prevalence rates may be due to differences in the socio-cultural factors of the studied populations and the different diagnostic methods employed.

The absence of any significant difference in the prevalence of HPV between the age groups in this study may be accounted for by the fact that the study population was predominantly young, 79.2% being 29 years or below. However, the peak age-specific prevalence of 11.7% which occurred in the 15-19 years age bracket and the high prevalence of 20.4% [30/293] in the 20-29 years age bracket is in keeping with the findings of other authors, which showed that the rates of cervical human papilloma virus infection were high in teenagers at the onset of their sexual experience [17] and in young adults who belonged to the most sexually active group.[12,17-19] This age range also corresponds to that of other sexually transmitted diseases like gonorrhea and non-gonococcal infections.[11]

Sexual attitudes may influence the occurrence of cervical human papilloma virus infection. Multiple sexual partners and coital frequency were the coital factors significantly associated with HPV infection in this study. These two factors, especially multiple sexual partners, increase the probability of having sexual contact with a male partner harboring the virus since not all men carry the agent. It is hence understandable why all the other sexual attitudes may turn out not to be associated with cervical HPV infection in the absence of multiple sexual partners. This is also the observation of other authors.[11,17-19] Since the duration of infectiveness of HPV is unknown and genital HPV is very common among persons who have been sexually active,[20] the absence of any significant association between HPV and past sexually transmitted diseases might be accounted for by the fact that most women are very reluctant to disclose the details of previously sexually transmitted diseases or their treatment.[16]

There was significant association between contraception, duration of contraception use, and cervical HPV infection. Hormones and their metabolites have been implicated as co-factors in HPV proliferation and the cervical cancer pathogenesis.[21,22] In their work, Von Kenbel Doeberitz, et al.[22] suggested an association between HPV infection and the long-term use of oral contraceptives in the genesis of cervical cancer. Progesterone- and estrogen-responsive elements were identified in the upstream regulatory region (URR) of HPV 18 transgenic mice and in other anogenital HPV types, particularly the high-risk types, which mediate the response of the enhancer to steroid hormones. Progesterone can also suppress the class I human leukocyte antigen surface expression in HPV-positive cervical cells.[23] The effect of contraception might also be secondary to multiple sexual partners due to the ‘safety’ proffered against pregnancy.[24] Those who had used contraceptives for ≥ 6 years had ten times risk than those using contraceptives for less than one year irrespective of the method used, probably buttressing increased sexual promiscuity with contraceptives. There was virtually total absence of use of barrier contraception and hence of any theoretical protection they might provide. Barrier contraceptives such as condoms are less likely to be effective in preventing infections such as genital HPV, which can involve skin not covered by a barrier contraceptive. Studies which have attempted to assess male condom benefit for women have generally found no evidence of protection against infection.[25-30] There is data suggesting a benefit of condom use for men although the studies have not adequately assessed consistency and correctness of condom use. Therefore, condoms should be used if only for prevention of other sexually transmitted diseases.[20]

Multiparous patients in this study stand a statistically significant risk of acquiring genital HPV infection compared to their non-parous counterparts. Multiparity probably reflects early sexual exposure. Early age of first pregnancy, early marriage, early sexual exposure, marital instability, and subsequent multiple sexual partners through remarriages or otherwise are all closely related with synergistic effects. They all increase the chances of acquiring sexually transmitted diseases, including HPV.[4,31] This is in agreement with the works of Schiffman, et al.,[32] and Lorenzato, et al.,[33] who found multiparity to be a risk factor for cervical HPV infection.

There was a statistically significant association between marital status, occupation, and cervical HPV infection. Among occupation groups, those with secured means of livelihood such as housewives (2.3%), business executives (3.7%), and civil servants (4.0%) were at least risk of HPV infection compared to students (37.5%), apprentices (33.3%), and petty traders (18.9%). The underlying factor might be multiple sexual partners as those who engage in sexual promiscuity do so for economic reasons.[11] Married women, probably due to marital stability with only one sexual partner hence less likelihood of exposure to a male carrier of HPV, were 25 times less likely to develop HPV infection of the cervix compared to single sexually active women who would probably have multiple sexual partners and have greater chances of encountering a male carrier of the infection. This is in keeping with the host donor agent theory,[34] which states that every act of coitus has a separate and fixed probability that one or more cells will become available to the carcinogen inoculated within the act of coitus. The significant association between religion and HPV infection is a study bias because almost all the population studied were Christians.

The significant association between cervical HPV infection and the clinical features of abnormal vaginal discharge, irregular menstruation, post-menopausal bleeding is in keeping with the sexually transmitted nature of the virus and its association with the pre-malignant cells and malignant lesions of the cervix as these clinical features are those of lower tract infection since HPV infection might be superimposed with bacterial infection.

This study will help to establish the prevalence of human papilloma virus infection of the cervix in the study population, thereby giving a measure of the potential for the development of pre-invasive cervical lesions in our environment as well as establishing the magnitude of the problem, especially in reference to identifiable epidemiological variables. It would also facilitate an early and appropriate management of such lesions. The study will also serve as part of a database for future studies on HPV infection in our environment. It is hoped that this work will re-kindle interest in the cytology of the cervix on the part of all concerned and facilitate the establishment of an organized cervical cytology program in our center. The required personnel and facilities to run an organized screening program are also available in our center.

Limitations

The two major limitations are that it is a hospital-/clinic-based study, and it is opportunistic. Because of both reasons, the patients may not necessarily represent the general population of women in the larger community. Being a fee-paying teaching hospital, there may be a larger concentration of more privileged and educated women that is representative of the community. Opportunistic screening also tends to be associated with a higher proportion of women of the young age group. In view of these, generalizations regarding the whole community must be made with caution. There is also a limitation in the methodology in that the ultimate diagnosis of HPV infection is based on the detection of viral DNA. Also, the relative contribution of each of the risk factors was not assessed. In addition, cytological diagnoses are fraught with errors. Errors can be made in the interpretation of the smears. Such errors may arise due to the presence of other genital tract infections causing, inflammatory cell exudation and cell degeneration, or cell reaction due to trauma or healing. These can result in false negative or positive results. There is also the problem of sensitivity, specificity, and HPV typing. These are what future researches on the subject will tend to explore.

Conclusion

There is a high prevalence rate of HPV infection among women attending the various clinics of the Department of Obstetrics and Gynecology of the Imo State University teaching hospital, Orlu. This is reflected even among teenagers. There is an association with multiple sexual partners, coital frequency, multiparity, contraceptive use, marital status, abnormal vaginal discharge, post-coital bleeding, and menopausal bleeding. These associations may allow for targeting of a high-risk group in an organized systematic cervical screening program, without discriminating other sexually active women.

This is a very cost-effective way of incorporating HPV testing in the current cervical screening programs in low resource settings such as ours where the newer and better methods of detecting genital HPV infection are not currently available. Multicenter research collaboration using these newer research methods that detect viral DNA is needed to further strengthen the validity of these associations.

The Null Hypothesis is hereby discarded, and the Alternate hypothesis adopted.

Source of Support: Nil.

Conflict of Interest: None declared.

References

Emerging Sources Citation Index (ESCI)
Emerging Sources Citation Index
Emerging Sources Citation Index
Indexed in

PubMed Central Index Copernicus Emerging Sources Citation Index
Abstracted/Indexed in

  • Include Baidu Scholar
  • CNKI (China National Knowledge Infrastructure)
  • EBSCO Publishing's Electronic Databases
  • Exlibris – Primo Central
  • Google Scholar
  • Hinari
  • Infotrieve
  • National Science Library
  • ProQuest
  • TdNet
  • African Index Medicus
Annals of Medical and Health Sciences Research The Annals of Medical and Health Sciences Research is a bi-monthly multidisciplinary medical journal. more >>
Submit your Manuscript