Background: Parents play a pivotal role in cultivating a positive dental attitude and healthy dietary and hygiene practices in their children if they have accurate knowledge about oral diseases and healthy dental practices. This study aims at assessing the knowledge, attitude, and practices of the parents of pre-schoolers in Saudi Arabia. Materials & Methods: A descriptive cross-sectional study has been conducted among the parents of 0-5-year-old children in Riyadh, Saudi Arabia. An online questionnaire was circulated among the participants containing 18 close-ended questions. Chi-square test, unpaired T-test, and ANOVA tests were computed to analyze the data using SPSS version 17 software (Illinois, Chicago, USA), wherein p-value<0.05 was considered statistically significant. Results: Overall, 205 parents participated in the study. Fathers scored a higher mean score (10.08) than mothers (9.65), and the comparison was not statistically significant (p>0.05). The education level did not significantly (p<0.05) impact parents with both high school and university level education (9.8). Parents of the 31-40 years age group scored higher mean scores (10.36) while 51-60 years age group achieved the least mean score (9.12). There was no significant difference observed among and within the age groups (p>0.05). Conclusion: Fathers in this study showed high mean scores than the mothers while there is no difference in parent’s education and parents belong to 30-40 years had achieved good scores. There was no evidence of observed gender, education, and age on knowledge, attitude, and practices regarding oral hygiene among parents participated in the study.