Background: Regular usage of denture cleansers is recommended in complete denture wearers for effective plaque control, and these cleansers alter the physical properties of acrylic resin over a period of time. Thus, an in vitro study was carried out to assess the effect of denture cleansers on the color stability of heatâÂÂcure denture base resin. Aim: The aim of the present study was to evaluate the effect of commercially available fastâÂÂacting denture cleansers on the color stability of heatâÂÂcure denture base resin at different time intervals. Materials and Methods: ThirtyâÂÂsix heatâÂÂcure acrylic resin (Ivoclar Triplex HotâÂÂV) specimens are randomly allocated into four groups âÂÂ Group A (distilled water as control); Group B (polident âÂÂ 3 min); Group C (fixodent scope plus); and Group D (stain away plus) comprising of nine samples each. After recording the baseline values, the specimens were immersed in their respective cleansing solutions for a prescribed time interval. This procedure was repeated daily, and the color change (ΔE) was evaluated after 90 and 180 days interval using a colorimeter in a standard “Commission International de l’Eclairage” color system. Statistical Analysis Used: Paired tâÂÂtest and Dunnett’s T3 test. Results: All the groups exhibited a variable color change (ΔE) for an immersion period of 90 days. However, significant color differences (P = 0.001) were noticed among the test groups after 180 days. Conclusion: The color change of denture base resin was greater for Group D followed by Groups B, C, and A respectively after 180 days of immersion. The ΔE values of all test groups increased with time.