Comparative Evaluation of Biocompatibility of Madhuca Longifolia Saponin Seed Extract with Sodium Hypochlorite-In Vitro Study

Author(s): Aishuwariya T and Sindhu Ramesh*

Background: Success of a root canal therapy depends on how well a clinician eradicates the microorganisms from the complex root canal system. Synthetic irrigants have been associated with harmful side effects. Herbal products with minimal side effects are gaining importance.
Objectives: To evaluate the biocompatibility of novel irrigant from Madhuca longifolia seed extract on the L929 cell lines.
Methods: The extract was prepared by using 10 gm of the powdered sample of Madhuca longifolia with 85% methanol using Soxhlet extraction method. L929 fibroblast cell lines were purchased and cells were cultured in a humidified atmosphere at 37°C in the cell growth Dulbecco's Modified Eagle medium. Cytotoxicity test was performed using MTT assay. The median lethal concentration of the test samples were expressed as the percentage survival of the cells. One-way analysis of variance (ANOVA) and post hoc least-significant difference test was done.
Results: Saponin extract showed 75% cell viability but Sodium hypochlorite showed a cell viability of 22%. Madhuca longifolia saponin seed extract at 300 and 400 micrograms concentration had a higher percentage of cell viability than the positive control, Sodium hypochlorite. Results were statistically significant with P value less than 0.05.
Conclusion:Madhuca longifolia seed extract has been proven to be more biocompatible than sodium hypochlorite. It is also proven to have good antibacterial properties. Madhuca longifolia has the potential to be used as an intracanal irrigant.


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