Background: Citrus sinensis is one of the most important and widely known fruits known for its medicinal value. Many medicinal properties of orange peel extract are known to fight viral and bacterial infections. ROS are oxygen-containing, chemically reactive molecules. ROS can kill bacteria but it also destroys the adjacent infected host tissues. The aim of the study was to determine the neutralizing effect of limonene (orange peel extract) on Reactive Oxygen Species (ROS) generated by the mixture when used as an intracanal medicament.
Methodology: Aqueous and ethanol extracts prepared from peel of Citrus sinensis were screened for neutralizing effect of limonene (orange peel extract) on Reactive Oxygen Species (ROS) generated by the mixture when used as an intracanal medicament. The groups were analyzed for ROS formation using the mass spectrometer (JEOL GC MATE II) immediately after preparation.
Results: The peak value of 3345.6 m/z denotes ROS formation. Group C shows a higher peak value than other groups.
Conclusion: The peak value of 3390.8 m/z was probably originated by the production of reactive compounds. Thus decreased ROS formation was noted in orange peel aqueous extract warranting further in vivo clinical studies to determine the exact dosages and its effectiveness in practical situations.