Clavicle, a collar bone also known as ‘Beauty bone’ is unique in its features as it is the only long bone which lies horizontally in humans. It has its own peculiarities in relation to ossification, as it has two primary centres, develop in membrane and it is the first bone to ossify. Various parameters like length, weight and midshaft circumference is studied in present study of both sex to assess the sex of that particular bone. The study was conducted at GMC, Nagpur from 2003 to 2006. The objective of this study to assess the sex of clavicle from unknown clavicle by taking certain medical parameters. This study is useful in medico legal and anthropometric study of clavicle.