Evaluation of Cytotoxicity of Gymnema sylvestre on L929 Mice Fibroblast Cell: An In Vitro Study

Author(s): Keerthika R and Sandhya Raghu*

Background: Cytotoxicity is defined as the toxicity caused due to the action of chemotherapeutic agents on living cells. Cytotoxicity tests are very important as they help in the determination of the proposed biomedical use. It has been proven that these cytotoxic properties of the medicinal plants are mainly due to its phytochemical constituents. Gymnema sylvestre is one such traditional ayurvedic herb used primarily for the treatment of diabetes and other ailments such as arthritis, anemia, diuretics, etc.
Aim: The aim of this study is to analyse the cytotoxic property of ethanolic extract of Gymnema sylvestre in comparison with the sodium hypochlorite.
Materials & methods: The ethanolic extract of Gymnema sylvestre was prepared and the cytotoxic potential of the extract at different concentrations (25,50,100,200 μg/ml) was tested on L929 Mice Fibroblast Cell using MTT assay and compared with that of the standards sodium hypochlorite (NaOCl).
Results: It is shown that the ethanolic extract did not adversely affect the fibroblasts even up to 50% concentration showing a nontoxic effect even till 200 μg/ml dose in comparison with NaOCl on these cells.
Conclusion: Within the limitations of this study it can be concluded that ethanolic extract of Gymnema sylvestre did not adversely affect the fibroblasts even up to 50% concentration showing a nontoxic effect even till 200 μg/ml dose in comparison with NaOCl on L929 Mice Fibroblast cells. However, further preclinical and ex vivo studies have to be carried to prove its use in clinical trials.


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