Introduction: In the treatment of Ayurveda medicines are prescribed to be taken with various media of intake like warm water, honey, ghee, milk etc. Such substances for Taking medicines are called as Anupana. While treating the diseases Anupana plays important role in the treatment.As in Ayurveda there are six different anupana with Haritaki has been mentioned by acharya Bhavamishra.Haritakiprovides the rasayana karma (restorative effect)by cleansing the channels of body. Objective: To evaluate the bioavaibality of Haritaki with Sharkara as anupana& to evaluate bioavailability of Haritaki without Sarkara. Methodology: Total 60 patientwere selected from swastharakshanopd of MGACH & RC. Then Haritaki plant and sharkara will be procure from Authentic source. Haritakikwath will be given to patient with and without sharkara after that at a predetermined interval of 0, 30, 60, 90, 120, 180, 240, 300 and 360 min after administration of Haritaki, (2ml) blood will be collected from pre-inserted cannula in the vein and immediately transferred to the EDTA tubes.Finally, the HPLC separation carried out for decoction of dried fruits of Terminalia chebula Retz., along with its bioenhancer effect will be compared with plasma separated from selected healthy volunteers to evaluate the presence of active metabolites responsible for exhibiting antioxidant activities. Discussion and Conclusion: Will be drawn on the basis of results found.
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