Evaluation of the Secretion of Nerve Growth Factor by Dental Pulp Fibroblasts that have been Stimulated by Caries Related Bacteria

Author(s): Rukhsaar Akbar Gulzar*, Ajitha P and Haripriya Subbaiyan

Introduction: During inflammation of pulp, an elevation in the secretion of various inflammatory biomarkers and cytokines by fibroblasts have been observed. Nerve Growth Factor (NGF) is a protein that has been associated with neuropathic and nociceptive pain. Dental pain involves nociceptive pain mechanism due to the presence of A∂ (myelinated) and C (unmyelinated) fibres. Thus, highlighting the probability of the role of NGF signalling pathway in dental pain. The present study was conducted to evaluate the secretion of NGF by pulp fibroblast cells.
Materials & Methods: The human fibroblast cell lines were purchased from American Type Culture Collection (ATCC). All these fibroblasts were cultured in Dulbecco's Modified Eagle Medium (DMEM). A pure culture of test strain of S. mutans ATCC 29212 was inoculated in sterile nutrient broth. The human dental pulp fibroblasts were stimulated with varying concentrations of S. mutans. Live bacteria were added to the cells at the ratio (bacteria:cells) of 10:1; 50:1 to 100:1. The supernatant collected was subjected to ELISA test to evaluate the levels of NGF.
Results: An increase in the secretion of nerve growth factor by stimulated dental pulp fibroblasts was observed with increase in the concentration of S. mutans. Highest levels of NGF 68.2 pg/ml were secreted by cells subjected to bacteria in the ratio 100:1.
Conclusion: The elevation in secretion of NGF by stimulated dental pulp fibroblasts with caries related bacteria suggests the involvement of NGF signalling pathway during pulpal pain which could be used to develop new pharmacological approaches for effective pain management.


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