The total number of patients in this study was 200 (114 males (57%) and 86 females (43%)). The patients were categorized to teenagers (21,10.5%), adults (92,46%), middle age (25,12.5%) and elderlies (25,12.5%) according to age. Five Enterobacteriaceae strains were found: Enterobacter cloaca (7,3.5%), Escherichia coli (111,55.5%), Klebsiella pneumoniae (75,37.5%), Proteus mirabilis (6,3.0%) and Pseudomonas aeruginosa (1.0,0.5%). P. aeruginosa was absent in all female patients under investigation. The response of the isolated (E. coli, K. pneumoniae and E. cloaca) strains to ampicillin, cefotaxime, cefotaxime-clavulanate, ceftriaxone, cephalothin and chloramphenicol, ciprofloxacin, nalidixic acid, streptomycin, tetracycline and trimethoprim-sulphamethoxazole was highly resistant, while the response was highly susceptible to ampicillin sulbactam, ceftazidime, ceftazidime-clavulanate, and imipenem. The most frequent gene was blaCTX-M (195) followed by blaTEM (149), blaSHV (73) and blaOXA (3), while the highest pair of genes in the same organism was blaTEM+blaCTX-M (134) followed by blaSHV+blaCTX-M (64), blaSHV+blaTEM (52) and the least pairs were blaTEM+blaOXA (3) and blaCTX-M+blaOXA (2). blaSHV+blaCTX-M+blaTEM was found in 44 organisms and blaCTX-M+blaTEM+blaOXA in two organisms only. The blaSHV+blaOXA, blaSHV+blaCTX-M+blaOXA, blaSHV+blaTEM+blaOXA and blaCTX-M+blaTEM+blaOXA+blaSHV were not present in any organism under investigation. In conclusion, in teenager group, there were no organism contain blaOXA gene, while blaOXA present in E. cloaca and P. mirabilis only. blaSHV gene was absent in E. coli but present in E. cloaca and K. pneumoniae in this study. The most susceptible group to infection with Enterobacteriaceae was adults’ group, while teenage group was more resist to infection.