Objective: To associate the level of Physical fitness level in women with and without Polycystic Ovary Syndrome (PCOS). Methodology: This Analytical cross-sectional study was conducted in 113 females who were divided into two groups PCOS and NON-PCOS. Data were collected through quota sampling technique. Females of age range (18-45 years) were selected for the study. Outcome measure was valid and reliable GPPAQ (General Practice Physical Activity Questionnaire). SPSS version 22 was used for data analysis. Chi square test was used in analyzing the data. Results: Mean and standard deviation of age of females were 24.24 ± 4.207. Out of total sample size of 113 females, 92 females fill the questionnaire, 44 with PCOS and 48 with Non- PCOS. Among our subjects, level of physical fitness was inactive (17.4%), moderately inactive (39.1%), moderately active (31.5%) and active (12%). P-value of >0.001 showed no association between level of physical fitness and PCOS. Conclusion: The study concludes that level of physical fitness not associated with PCOS.