Background: Failure to conceive following regular or frequent unprotected intercourse for a year or more has been defined as infertility. In-Vitro Fertilization (IVF) is the most recommended treatment for unresolved infertility issues worldwide. There are various factors associated with the risk of infertility and if left intreated these may lead to an exaggerated damage that further results into recurrent IVF failure in these patients. Materials & Methods: This study was conducted at Krishna Medical Centre, Lucknow, Uttar Pradesh, from September 2012 to December 2019 in 800 patients experiencing infertility problem. The study includes females with valid indication for IVF treatment age between 20 years to 50 years. Various pathological, environmental and anatomical factors associated with infertility have been investigated in the patients. The surgical interventions used to modify the anatomical barriers leading to infertility in the patients have also been mentioned in the manuscript. Result: In this study it was found that there are various physical, physiological, environmental and anatomical risk factors that pose threat to successful pregnancy in the infertile couples. These factors also create restrictions in successful conception through in vitro ferlilization in these subjects. If left untreated these factors may lead to permanent sterility and infertility in these couples. Hence timely treatment and proper medical care along with surgical intervention can help the patients overcome their fertility issues via IVF. Conclusion: This study contains the data of 800 female patients suffering from various types of infertility issues. The investigators have studied the factors associated with infertility and have also tied to treat the anatomical barriers posing threat to fertility in these subjects. The article is being produced with the hope to generate awareness to the infertile couple with knowledge and information of the medical advancements that can benefit them in overcoming their infertility issue and lead a successful life.