Pseudoexfoliation Syndrome (PXS) is a disorder characterized by theprogressive accumulation of fibrillary extracellular deposits in several oculartissues. It is an independent risk factor for glaucomatous optic nervedamage. Retinal Nerve Fiber Layer (RNFL) thickness analysis using opticalcoherence tomography is a documented investigative tool to detectglaucoma at an early stage. The aim was to evaluate and compare RNFLthickness in PXS patients without glaucoma with their age and sex matchedhealthy controls and to detect the possibility of early glaucomatous damagein patients with RNFL thinning. A total of 100 patients were included, ofwhich 50 were cases (Group A) and 50 were controls (Group B). RNFLthickness of cases and controls were compared using Zeiss Cirrus HD-OCT500.