Background: Computer Vision Syndrome (CVS) described by the American Optometric Association (AOA) as “The complex of eye and vision problems related to near work experience during computer use.” The current study aims to evaluate the prevalence of CVS and the association between CVS symptoms with age and behavior’s, among college students and employees in Jeddah, Saudi Arabia. Subjects & Methods: This was a cross-sectional analytic study conducted in Jeddah, Saudi Arabia from July to August 2020. The study targets college students and employees living in Jeddah, Saudi Arabia, from July 2020. The target sample size was a minimum of 377 that was calculated using raosoft. Data entry was performed by Microsoft Excel 2019, and analysis was done using Statistical Package for the Social Sciences (SPSS) software, version 25. Frequency, descriptive analysis, Chi-square, and One-way ANOVA was performed. P-value of <0.05 was considered statistically significant. Results: Overall, 786 responses were received, only 459 met the criteria of which 277 (60.3%) were college students and 182 (39.7%) were employees. Pain in and around the eyes 288 (66.5%) and sore eyes 247 (57%) were the most commonly reported ocular symptoms among participant and in terms of extra-ocular symptoms; headache 355 (82%) and neck and shoulder pain 237 (55%) recorded highest. The highest number of symptoms was reported by IbnSina National College (ISNC) students with approximately a mean number of symptoms 7 ± 3.443. Conclusion: Computer Vision Syndrome is a common syndrome among college students and employees.