Interest in medicinal plants has burgeoned due to increased efficiency of new plantderived drugs and the growing interest in natural products. Because of the concerns about the side effects of conventional medicine, the use of natural products as an alternative to conventional treatment in healing and treatment of various diseases has been on the rise in the last few decades. The use of plants as medicines dates from the earliest years of man’s evolution. Medicinal plants serve as therapeutic alternatives, safer choices. A larger number of these plants and their isolated constituents have shown beneficial therapeutic effects so we worked. This study to determine the effect of different levels of pumpkin seeds on rats injected with carbon tetrachloride (CCl4). The experiment was performed in animal house. All rats were fed for one week on basal diet before starting the experiment, then divided into two main groups, the first group (n= 6 rats) was fed on the basal diet only as a control negative (C –ve ) normal rats for 28 days. The rats of second main group (n= 24 rats) were injected with carbon tetrachloride (CCl4), The second main group is divided into four sub-groups, including 3 groups fed with different concentrations of (5%, 10%, and 15%) pumpkin seeds and one group control positive infected with the disease do not feed on the experimental diet. the results showed that there is no significant difference between the values of ALP enzyme of group 3 and control positive group. Also, there is no significant difference between groups 4, and 5 showed similar (P>0.05. There is no significant difference between group 5 and control positive group. Also,. Groups 3, and 4 showed similar (P>0.05) mean values of ALT. Also there is no significant difference between group 3 and control positive group. Also, group 4 was similar (P>0.05) to normal group. Group 5 showed lower (P<0.05) urea nitrogen than both control groups.