Research Article
Investigation of 13q14.3 Deletion by Cytogenetic Analysis and FISH Technique and miRNA-15a and miRNA-16-1 by Real Time PCR in Chronic Lymphocytic Leukemia
Author(s):
Melike Yılmaz, Dilhan Kuru R*, Isil Erdoğan, Teoman Soysal, Seniha Hacıhanefioglu and Onur Baykara
Background:The most frequent cytogenetic aberration is 13q14.3 deletion in Chronic Lymphocytic Leukemia (CLL). Hsa-miR-15a/hsa-miR-16-1 are tumor suppressor miRNAs encoded from 13q14.3 region. Objectives: The aim of this study was to investigate the 13q14.3 deletion using molecular and cytogenetic techniques and association with miRNA-15a/miRNA-16-1. Materials & Methods: We used peripheral blood samples of 30 CLL patients which were either induced and or non-induced with DSP30+IL-2 for determine 13q14.3 deletion by karyotyping and iFISH methods. Expression levels of hsa-miR-15a/miR-16-1 were measured using Quantitative Real Time PCR and compared with deletions. Results: 13q14.3 deletion was detected in 8.6% of cases by karyotyping and in 65% by iFISH. Mosaic forms (monoallelic+biallelic) were observed in 50% of cases. Besides determining common chromosome abnormalities such as add.. Read More»
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