Aim: The aim of this study was to investigate the antibiotic resistance of staphylococci and seek toxin production by Staphylococcus aureus strains isolated from urogenital infections. Material and Methods: The staphylococci strains were isolated from urogenital samples collected from hospitalized patients or not. The antibiotic susceptibility was performed by the diffusion method and the search of production of toxin by S. aureus was done by radial immunoprecipitation technique. Results: Out of the 1904 samples analyzed, 80 staphylococci strains were isolated. The major (70%) part of the positive samples were coagulase-negative staphylococci composed of S. saprophyticus (50.0%), S. epidermidis (16.25%), S. xylosus (2.5%), and S. haemolyticus (1.25%). S. aureus was the unique coagulase positive strains. It was observed a multi-resistance of the isolated strains to beta-lactams, aminoglycosides, tetracycline, and co-trimazole. All the S. haemolyticus and S. xylosus strains were resistant to methicillin. Nitrofurantoin was the most active molecule in all kind of strains. There was no methicillin-resistant S. aureus producing Panton-Valentine Leukocidin (PVL) detected but all the S. aureus producing PVL were community methicillin-sensitive S. aureus. Most of the tested strains produced ETB (83.33%) and ETA (45.33%). Conclusion: The presence of multidrug resistance staphylococci strains producing toxins indicate an existence of potential reservoir of virulent antibiotics resistance stains in the community.