Background: Gum Arabic (Acacia) is an edible dried gummy exudate from the stems and branches of Acacia Acacia and A Seyal. The biologic effects of gum Arabic includedthe enhancement of dental tissue remineralization due to its rich content of calcium, magnesium, and potassium salts. The aims of the current study were directed to evaluate the clinical effect of (Acacia) on the reduction of root dentine hypersensitivity and to compare the impact of acacia with the potassium nitrates (Kno3) clinically. Subjects & Methods: Randomized, double-blind, controlled clinical trial using splitmouth design tested on 52 teeth selected from 26 female patients with a mean age of 37 years that had hypersensitivity following non-surgical periodontal therapy through root planing. Candidates were chosen randomly from the periodontics division, college of dentistry, King Khalid University (KKU). The Verbal Rating Scale (VRS), was applied to evaluate the degree of hypersensitivity based on the patient’s response to tactile and air blast stimuli, recorded from (0 cm to 10 cm). In addition, a Visual Analog Scale (VAS). Test was employed to record pain intensity. Group A (n=26 teeth surfaces) received acacia gel, which applied topically to the sensitive root surfaces, and group B (n=26 teeth surfaces) treated by KNO3 on teeth of the contralateral side, control group. Both groups received consecutive applications at days (1st, 7th and 14th). Results: Air blast scale showed pain reduction on both test and control groups immediately after the treatment, one week and 14 days following application. There is no statistical significant difference in the reduction of root sensitivity between acacia and KNO3 groups (P-Value<0.05). Conclusion: Arabic gum or acacia effectively reduces the pain of hypersensitive root dentine following root planning without any side effects. Acacia and KNO3 are equally effective in lowering root dentine hypersensitivity.