Background: The 10âitem version of Perceived Stress Scale (PSSâ10) is a widely used tool to measure stress. The Malay version of the PSSâ10 has been validated among Malaysian Medical Students. However, studies have not been conducted to assess its validity in occupational settings. Aim: The aim of this study is to assess the psychometric properties of the Malay version of the PSSâ10 in two occupational setting in Malaysia. Subjects and Methods: This study was conducted among 191 medical residents and 513 railway workers. An exploratory factor analysis was performed using the principal component method with varimax rotation. Correlation analyses, KaiserâMeyerâOlkin, Bartlett’s test of Sphericity and Cronbach’s alpha were obtained. Statistical analysis was carried out using statistical package for the social sciences version 16 (SPSS, Chicago, IL, USA) software. Results: Analysis yielded two factor structure of the Malay version of PSSâ10 in both occupational groups. The two factors accounted for 59.2% and 64.8% of the variance in the medical residents and the railway workers respectively. Factor loadings were greater than 0.59 in both occupational groups. Cronbach’s alpha coâefficient was 0.70 for medical residents and 0.71 for railway workers. Conclusion: The Malay version of PSSâ10 had adequate psychometric properties and can be used to measure stress among occupational settings in Malaysia.