Background: Resin bonded bridges (RBBs) are cost-effective, simple to use, and minimally-invasive compared to other fixed options for the replacement a short span or single tooth. Objective: The aim of this study was to assess the knowledge, attitude, and practice of undergraduate senior dental students (USDSs) and general dental practitioners (GDPs) toward resin bonded bridges in the Asir region of Saudi Arabia. Methods: In this cross-sectional study, a questionnaire was used to assess the knowledge and practice of RBBs. The attitudes of the participants toward continuous education were evaluated. Chi-square and Fisher’s exact tests were used to compare the responses of the two groups of participants. Results: The overall response rate was 78.9% (300/380). Less than half of the participants in both groups considered RBB as a successful conservative approach for restoring missing teeth. More than 50% of both the USDSs and GDPs selected fixed–fixed as the most successful RBB design. Only half of the participants demonstrated confidence in offering RBBs to their patients due to lack of education and training regarding the use of RBBs. About 50% of the GDPs and 25% of the USDSs were exposed to the process of replacing missing teeth using RBBs during their clinical undergraduate studies. Conclusion: The lack of skill and training on the use of RBBs was consistent in both groups. Improvements in the undergraduate dental curriculum and clinical skills of the students and continuing education opportunities for the GDPs are required to increase their exposure to RBBs.