Original Article
Investigate the Inhibitory Effects of Satureja khuzestanica Essential Oil against Housekeeping fabD and exoA Genes of Pseudomonas aeruginosa from Hospital Isolates using RT-PCR Technique
Author(s):
Asieh Abbasi, Sayyedeh Mahdokht Maddah, Arash Mahboubi, Azad Khaledi, Hossein Vazini and Davoud Esmaeili*
Background: Pseudomonas aeruginosa is an opportunistic pathogen which causes a range of infections. Satureja khuzestanica essences have antibacterial effect. Aim: This study aimed to investigate the inhibitory effects of S. khuzestanica essential oil against housekeeping fabD and exo A genes of Pseudomonas aeruginosa from hospital isolates using RT-PCR technique. Material and Methods: The inhibition effect was conducted according to the CLSI 2013 standards and instructions. The minimum inhibitory concentration (MIC) of essence was determined according to CLSI. Isolates were collected of burn patients in the Motahari hospital, Tehran and cultured on proper media and confirmed using microbiology and biochemical. Susceptibility and MIC of S. khuzestanica were determined against P. aeruginosa. Expressions of e.. Read More»
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